Figure 3
From: Encapsulation of gold nanoparticles into self-assembling protein nanoparticles
Encapsulation of 10 nm citrate-coated GNPs into P6c SAPNs. TEM images of the encapsulation samples that have three different ratios of P6c protein to GNPs, respectively: (a) The P6c protein concentration was 0.05 mg/ml (approximately 4 nmol/ml). The 10 nm GNPs concentration was 4.7×10-4 nmol/ml (0.47 nM). (b) The P6c protein concentration was 0.05 mg/ml. The 10 nm GNPs concentration was 4.7×10-3 nmol/ml (4.7 nM). (c) The P6c protein concentration was 0.025 mg/ml. The 10 nm GNPs concentration was 4.7×10-3 nmol/ml (4.7 nM). The 10 nm GNPs were first diluted in the refolding buffer containing 10 mM HEPES, 75 mM NaCl, 5% glycerol, pH 7.5. Then the denatured P6c protein was refolded in the GNPs solutions using the quick refolding method. After protein refolding, all three samples were dialyzed in a buffer containing 20 mM HEPES, 150 mM NaCl, 5% Glycerol. (d) DLS profiles for volume distribution of hydrodynamic sizes of the encapsulation samples.