|
Groups
|
N
|
Motility1(%)
|
Rapid2(%)
|
VAP (μm/sec)
|
VCL (μm/sec)
|
VSL (μm/sec)
|
|---|
|
Control*
|
5
|
87 ± 6a
|
74 ± 5
|
94 ± 11
|
202 ± 23a
|
56 ± 9
|
|
0.1 x 108
|
3
|
36 ± 6b
|
23 ± 13
|
40 ± 5
|
86 ± 15b
|
21 ± 5
|
|
0.5 x 108
|
4
|
63 ± 17ab
|
46 ± 15
|
65 ± 19
|
143 ± 36ab
|
39 ± 12
|
|
1 x 108
|
4
|
81 ± 8a
|
68 ± 11
|
90 ± 9
|
194 ± 17a
|
51 ± 7
|
|
2 x 108
|
3
|
89 ± 5a
|
63 ± 22
|
94 ± 17
|
198 ± 36a
|
52 ± 8
|
|
P values (ANOVA 2)
|
0.017
|
0.094
|
0.069
|
0.053
|
0.116
|
- *Spermatozoa incubated without BRET-QD (1 nM). N = number of independent replicates; 1Total motility of spermatozoa; 2Proportion of sperm moving at a speed ≥ 30 μm/sec. Velocity data correspond to the average path (VAP), curvilinear (VCL), and straight-line (VSL). Data are mean ± s.e.m. and superscripts (a,b) indicate significant differences within the same column.
