Figure 6

Effect of endocytic inhibitors on gene expression of P/G/L nanoparticles. Interferon (IFN)-γ expression was measured at 72 hours in cells incubated with P/G/L nanoparticles in the presence of endocytic inhibitors. In the P/12-7NH-12/L-treated cells, gene expression in cells pre-treated with caveolae-mediated uptake inhibitors (genistein, filipin) was significantly lower than the untreated (no inhibitors) cells, whereas gene expression was significantly higher on inhibiting clathrin-mediated uptake (chlorpromazine). For the P/12-7NGK-12/L nanoparticles, gene expression was significantly lower in presence of methyl-β-cyclodextrin (clathrin- and caveolae-mediated uptake inhibitor). * represents significant difference, p < 0.05, compared to 'No inhibitors' control cells treated with P/12-7NH-12/L nanoparticles (No inhibitors black bar). $ represents significant difference, p < 0.05, compared to 'No inhibitors' control cells treated with P/12-7NGK-12/L nanoparticles (No inhibitors dashed-line bar).