Figure 1

Effects of serum on actomyosin motility with and without magnetic separation to remove serum components. Full symbols and lines: with magnetic separation. One control experiment but at least twenty filaments for each velocity measurement. Dashed symbols and lines: without magnetic separation. Number of independent experiments in parentheses. Fraction of motile filaments (red; left vertical axis), average sliding velocity (blue; right vertical axis) and the fraction of experimental occasions with any motility at all (black) when the flow cells were directly incubated with blood serum at different dilutions. Sliding velocity (blue dashed line and open circles; data adapted from [22]). Error bars show SEM.