Figure 4
From: Magnetic capture from blood rescues molecular motor function in diagnostic nanodevices
Effects of magnetic pre-concentration and removal of serum. (a) The total number of filaments (grey bars) and the number of motile filaments (red bars) at the end of the procedure in Figure 3 for different concentrations of blood serum (0 – 100%) and in the presence (+) and absence (-) of antigen (r-IgG) in step I in Figure 3. (b) Fluorescent images from the experiment in (a) with or without antigen. In the presence of antigen (rIgG) several fluorescent filaments can be seen suggesting crosslinking between arIgG antibodies conjugate on actin and MPs via the antigen. In the absence of antigen only a few filaments are bound to HMM on the surface. Both images are from samples with 100% serum. Scale bar 10 μm.