Figure 2
Effect of nanostructured TiO 2 surface on neurite formation in PC12 cells. (A-H) Phase contrast photographs 10X magnification, Bar = 50 μm. PC12 cells were cultured for 48 h in low serum medium (1% horse serum) only (A, C, E and G) or with 50 ng/mL NGF (B, D, F and H) on four kinds of substrates: G-PLL (A, B), flat TiO2(C, D), ns-TiO2 20 nm rms (E, F) and ns-TiO2 29 nm rms (G, H). (I-L) Histograms of the neurite length mean and differentiation percentage. Histograms of the neurite length mean (I) and differentiation percentage (L) for each condition shown in A-H. G-PLL + NGF elongation length mean and differentiation values are not statistically significantly different from ns-TiO2 20 nm rms and ns-TiO2 29 nm rms. * p < 0.05, ** p < 0.01, one-way ANOVA with Tukey’s post hoc test.