Figure 6
Effect of iNOS inhibitors on neurite formation in PC12 cells induced by nanostructured TiO 2 surface. (A-E): Phase contrast photographs 20X magnification, Bar = 50 μm. PC12 cells were cultured on PLL-coated coverslips for 48 h in low serum medium (1% horse serum) only (A), with 50 ng/mL NGF (B) or with 50 ng/mL NGF and 2 mM SMT (C). PC12 cells were cultured on ns-TiO2 (20 nm rms) for 48 h in low serum medium (1% horse serum) only (D) or with 2 mM SMT (E). (F,G): Histograms of the neurite length mean and differentiation percentage in the presence of iNOS inhibitor. Histograms of the neurite length mean (F) and differentiation percentage (G) in the presence of iNOS inhibitor (2 mM SMT) for each condition shown in A-E. ** p < 0.01, one-way ANOVA with Tukey’s post hoc test.