Figure 5
From: Polyamidoamine dendrimer impairs mitochondrial oxidation in brain tissue
Ψ MITO depolarization after G5-NH 2 (0.1 mg/ml, 30 min) application is comprised of neuronal activity-dependent and independent astroglial components. Neuronal activity was blocked by TTX (1 μM), APV (100 μM), CNQX (10 μM) and picrotoxin (100 μM). Characteristic ΨMITO depolarization parameters, e.g. the number (A), length (B) and normalized fluorescence changes (C) of astroglial cells and neurons are shown. Fluorescence changes (ΔF/F0) were normalized to ΨMITO depolarization evoked by the mitochondrial inhibitor CCCP (10 μM). Asterisks represent significant differences at p < 0.05 level.