Figure 3

Example of an actin polymerization wave in Dictyostelium discoideum. Circular wave expanding the cell border of Dictyostelium discoideum. Cells expressing mRFP-LimED to label filamentous actin structures and GFP-Arp3 for incorporation into the Arp2/3 complex were recorded by dual-emission TIRF microscopy during recovery from latrunculin A treatment. The protrusion stops when the wave starts to collapse. Time is indicated in seconds. Scale bar: 10 μ m. From Bretschneider et al. [20] with permission from Biophysical Journal.