Figure 3

Representative images from coated and uncoated scaffolds. (a) 40× bright field image of uncoated scaffold and (b) fluorescent image of thymosin β4 coated scaffold. Thymosin β4 autofluoresces and can therefore be seen without use of GFP or fluorescein. Notice thickness change increase from uncoated to coated scaffold fibers. Scale bar: 100 μm. Images of cells on coated fibers at (c) 0.5%, (d) 1%, and (e) 1.5% thymosin β4. Significances in cell count are noted. *p < 0.05 by t-test n = 5; ns: no significance. Nanoscaffolds are labeled with blue arrowheads; cell bundles with red arrowheads.