Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles

Table 1 Mobility of fluorescent canine parvovirus recombinant proteins.

Particle	τ_D1 (ms)	τ_D2 (ms)	D 1 (m²s^-1)	No urea, Rh_D1 (nm)	6 M urea, Rh_D2 (nm)	Nf
EGFP-VP2	0.7 ± 0.3	0.3 ± 0.1	1.4 × 10^-11	17.0 ± 7.0	8.5 ± 2.7	9.5 ± 0.7
EGFP-VP2-14	0.3 ± 0.0	0.2 ± 0.1	3.6 × 10^-11	6.7 ± 0.9	3.9 ± 2.3	2.0 ± 0.1
EGFP-VP2-23	0.8 ± 0.2	0.4 ± 0.1	1.2 × 10^-11	20.0 ± 4.0	10.0 ± 2.5	5.8 ± 1.3
EGFP-VP2-40	0.5 ± 0.1	0.4 ± 0.0	1.8 × 10^-11	14.0 ± 3.4	9.0 ± 0.7	9.7 ± 0.1
EGFP	0.1 ± 0.0	0.1 ± 0.0	1.2 × 10^-10	2.0 ± 0.1	2.0 ± 0.1	1.0 ± 0.0

The diffusion times before (τ_D1) and after (τ_D2) treatment with 6 M urea at 50°C, diffusion coefficient (D 1), hydrodynamic radii in the absence (Rh_D1) and presence (Rh_D2) of 6 M urea at 50°C, as well as, the number of fluorescent moieties (Nf) present in the EGFP-VP2, EGFP-VP2-14, EGFP-VP2-23 and EGFP-VP2-40 fluorescent proteins and VLPs. The enhanced green fluorescent protein (EGFP) served as a reference.

ISSN: 1477-3155