|
Particle
|
τD1 (ms)
|
τD2 (ms)
|
D 1 (m2s-1)
|
No urea, RhD1 (nm)
|
6 M urea, RhD2 (nm)
|
Nf
|
|---|
|
EGFP-VP2
|
0.7 ± 0.3
|
0.3 ± 0.1
|
1.4 × 10-11
|
17.0 ± 7.0
|
8.5 ± 2.7
|
9.5 ± 0.7
|
|
EGFP-VP2-14
|
0.3 ± 0.0
|
0.2 ± 0.1
|
3.6 × 10-11
|
6.7 ± 0.9
|
3.9 ± 2.3
|
2.0 ± 0.1
|
|
EGFP-VP2-23
|
0.8 ± 0.2
|
0.4 ± 0.1
|
1.2 × 10-11
|
20.0 ± 4.0
|
10.0 ± 2.5
|
5.8 ± 1.3
|
|
EGFP-VP2-40
|
0.5 ± 0.1
|
0.4 ± 0.0
|
1.8 × 10-11
|
14.0 ± 3.4
|
9.0 ± 0.7
|
9.7 ± 0.1
|
|
EGFP
|
0.1 ± 0.0
|
0.1 ± 0.0
|
1.2 × 10-10
|
2.0 ± 0.1
|
2.0 ± 0.1
|
1.0 ± 0.0
|
- The diffusion times before (τD1) and after (τD2) treatment with 6 M urea at 50°C, diffusion coefficient (D 1), hydrodynamic radii in the absence (RhD1) and presence (RhD2) of 6 M urea at 50°C, as well as, the number of fluorescent moieties (Nf) present in the EGFP-VP2, EGFP-VP2-14, EGFP-VP2-23 and EGFP-VP2-40 fluorescent proteins and VLPs. The enhanced green fluorescent protein (EGFP) served as a reference.
