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Table 1 Mobility of fluorescent canine parvovirus recombinant proteins.

From: Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles

Particle τD1 (ms) τD2 (ms) D 1 (m2s-1) No urea, RhD1 (nm) 6 M urea, RhD2 (nm) Nf
EGFP-VP2 0.7 ± 0.3 0.3 ± 0.1 1.4 × 10-11 17.0 ± 7.0 8.5 ± 2.7 9.5 ± 0.7
EGFP-VP2-14 0.3 ± 0.0 0.2 ± 0.1 3.6 × 10-11 6.7 ± 0.9 3.9 ± 2.3 2.0 ± 0.1
EGFP-VP2-23 0.8 ± 0.2 0.4 ± 0.1 1.2 × 10-11 20.0 ± 4.0 10.0 ± 2.5 5.8 ± 1.3
EGFP-VP2-40 0.5 ± 0.1 0.4 ± 0.0 1.8 × 10-11 14.0 ± 3.4 9.0 ± 0.7 9.7 ± 0.1
EGFP 0.1 ± 0.0 0.1 ± 0.0 1.2 × 10-10 2.0 ± 0.1 2.0 ± 0.1 1.0 ± 0.0
  1. The diffusion times before (τD1) and after (τD2) treatment with 6 M urea at 50°C, diffusion coefficient (D 1), hydrodynamic radii in the absence (RhD1) and presence (RhD2) of 6 M urea at 50°C, as well as, the number of fluorescent moieties (Nf) present in the EGFP-VP2, EGFP-VP2-14, EGFP-VP2-23 and EGFP-VP2-40 fluorescent proteins and VLPs. The enhanced green fluorescent protein (EGFP) served as a reference.