Figure 4

C₆₀ does not inhibit cell proliferation. MCF 10A and (Panel A) MDA MB 231 (Panel B) cell lines were cultured either in the absence or presence of methanol C₆₀ (0.2 mg/ml) and cell proliferation was assayed by crystal violet staining. ◆ Control, no C₆₀, ■ 10 μg C₆₀, ▲ 50 μg C₆₀, X 250 μg C₆₀. (Panel C). MDA MB 231 cells were simultaneously stained with calcein and ethidium using a live-dead assay kit. Lack of red-colored cells and the presence of cells stained in green indicate the lack of toxicity (Panel D). MDA MB 231 cells were either untreated (open box □) cultured with varying amounts 10 (gray ), 50 (patterned ) and 100 μg (filled ■) of C₆₀ for 48 h and analyzed for cell cycle progression by flow cytometry.