Representative cryo electron micrographs (Cryo-EM) of buckysomes. Both unilamellar and multilamellar vesicles are seen. The scale bars in A, B, C, are 100 nm; D, E are 200 nm. Image C is a 45° tilt of B. The bilayer diameter is ~6.5 nm. Buckysomes were prepared in 10 mM citrate at pH 7.0 at a concentration of 2.0 mg/mL. (see Methods for detailed methodology on sample preparation).