Uranyl acetate negative-stained transmission electron micrographs (TEM) of various supramolecular structures of AF-1. Combined morphologies of rod-like, branched and elongated micelles are seen in addition to buckysomes. The scale bar is 100 nm in all the images. In micrographs (A, B, C) AF-1 was prepared in 10 mM HEPES at pH 8.0; in (D, E, F) AF-1 was prepared in 0.2 M phosphate-citrate and in (G, H, I) in 1 × PBS buffer at pH 7.15. The concentration of AF-1 was 2 mg/mL and preparations were made at room temperature. Images are representative of 20–30 different areas on the grid.