Figure 7

Anti-permeability effect of Ag-NP is reversed by over-expression of constitutively active Src kinase. PRECs were transiently transfected with dominant negative Src (HA-Src KD K295 M) and constitutive active Src (HA-Src-CA Y527F). Transfected PRECs were grown to confluent monolayers on porous membranes and then incubated with or without growth factors and Ag-NP for 6 h at 37°C, where the block in permeability by Ag-NP in wild type cells was overcome in CA-Src transfected cells. Ag-NP was added 30 min prior to growth factor. The flux of RITC-dextran from the upper to the lower chamber was measured 6 h after treatment. Values are expressed in relative fluorescence counts (RFCs) as mean ± SEM, with each condition performed at least in triplicate.