Figure 9

Src modulates the inhibitory action of Ag-NP on VEGF-and IL-1β-induced Src phosphorylation (Y419). PRECs were transiently transfected with dominant negative Src (HA-Src KD K295 M) and constitutive active Src (HA-Src-CA Y527F). (A) Shows the effect of Src mutants on VEGF-and IL-1β-induced Src phosphorylation. DN Src mutant significantly blocks the VEGF-and IL-1β-induced Src phosphorylation whereas CA Src had an additive effect on Src phosphorylation. (B) Shows the Ag-NP rescues the inhibitory effect of Src phosphorylation in CA Src mutant. CA Src mutant confers resistance to Ag-NP blocking effect on VEGF-and IL-1β-induced Src phosphorylation at Y419. Data are means ± SEM representing similar results was obtained in three independent experiments (n = 3, *P < 0.05 vs control, **P < 0.01 vs control).