Figure 4

Increased NIR-QDot size imposes constraints on Akt-PH-QD conjugate translocation efficiency but NIR-QD's allow visualization in deeper cell layers in a live Xenopus embryo, unlike Akt-PH-EGFP (a) Co-localization of QDot ₇₀₅ with Akt-PH-EGFP on the cell membrane. Note that unlike the QDot₅₈₅, the QDot₇₀₅ are not recruited as effectively to the cell membrane. (b) QDot₈₀₀ allow visualization of the Akt-PH ~ two to three layers below the superficial cell layer, where the GFP signal was either undetectable or too diffuse. The images are of the same region of the embryo imaged with a GFP (left) and a QDot₈₀₀ (right) filter set.