Figure 5

S segment quantitative real time PCR analysis. TCRV was treated with uncoated and PS-coated 10 and 25 nm Ag-NPs for 1 hour. Treated or control virus was used to infect Vero cells for 4 days. The supernatant was removed and the cells were washed 2 times with PBS to remove non-adherent virus. RNA was isolated from the Vero cells and S segment gene expression was determined using qRT-PCR with Sybr green. Changes in S segment gene expression are expressed as fold change over untreated TCRV infection with error bars representing the S.E.M. and statistical analysis comparing the untreated control to each of the treatment groups. (*p < 0.05; student's t test; n = 6).