Figure 4

Sustained delivery of nile red by PLGA-PEG nanoparticles. The nile red loaded PLGA-PEG nanoparticles were insufflated in Cftr^+/+ (n = 4) mice airway. Live animals were imaged by Xenogen IVIS 200 optical imaging device (Ex 465 nm and Em 525 nm) from day 1 to 11. All animals were kept under constant supply of isoflurane using an automated anesthesia machine attached to imaging device and handled in accordance with our JHU ACUC approved animal protocol. We observed significant amount of PLGA-PEG^{PS341-NileRed} particles in murine lungs and bladder (excreted nanoparticles) by 24 hrs and observed its sustained release from days 1 to 11 given the short half-life of the nile red.