Figure 5
PLGA-PEG mediated cystosolic delivery. The indicated concentrations of PLGA-PEGPS341-NileRed was added to CFBE41o- cells and incubated for 24 hours. Cells were fixed with 10% neutral buffer formalin and stained with Hoechst dye for nuclear staining. Fluorescence microscopy was used to capture images of Hoechst staining (DAPI filter) and nile red (Texas Red filter) that shows perinuclear cytosolic localization of released dye. We show the cytosolic release of nile red in perinuclear space using the PLGA-PEG nanoparticles containing 1000 or 2000 ng dye. The nile red dye added directly to the media at similar concentrations as a negative control did not show any cytosolic delivery after 24 hrs. The data verifies the efficacy of our novel therapeutic vehicles for bronchial epithelial cell delivery.