Figure 3

Measurement of the relative iron-loading of cells. (A) NIRF signal of cells labeled with IRDy800CW-MNPs at doses of 0, 20, 30, 40, 50, 60, 80 and 100 μg/ml (Column 1-8). Each dose contained 6 repeats (Row 1-6). Cells were washed with PBS before imaging. (B) Measurement of the relative iron-loading of cells (A) with colorimetric and NIRF approaches. Row 1-3: NIRF signals; Row 4-6: Colorimetric signals. (C) The intensity of colorimetric and NIRF signals (B). (D) The normalized intensity of colorimetric and NIRF signals (C). The signal of the nanoparticle-labeled cells was normalized to that of the negative control cell. The error bars represent mean and standard deviations of experiments performed in triplicate.