Figure 1

Design of the dual-label time-resolved immunofluorometric assay. (A) A schematic illustration of the assay for simultaneous detection of HIV and HBV infections. The Arabic numerals indicate individual assay components: (1) microtitre well surface; (2) streptavidin; (3) r-Bio-HIV-1 Env; (4) Bio mAb 21B; (5) anti-HIV-1 antibodies in infected serum; (6) HBsAg in infected serum; (7) r-HIV-1env labeled with Tb³⁺ chelate (which is measured at 545 nm); (8) 5 S F(ab)₂ coated Eu³⁺ nanoparticles (which is measured at 615 nm). (B) The emission spectra of Tb³⁺ chelate (green line) and Eu³⁺ nanoparticles (orange line).