Immobilization of glutathione-S-transferase (GST) on syntaxin beads. (A) Coomassie-stained gel showing that the GST-brevin fusion protein binds to the syntaxin beads, but not control beads. Binding of GST-brevin occurs via the SNARE complex, as indicated by the presence of SNAP25. (B) Graph showing kinetics of the specific GST activity attached to syntaxin beads measured by the increase in absorbance at 340 nm due to conjugation of glutathione to 1-chloro-2,4-dinitrobenzene. The data show mean +/- standard deviation, n = 3.