Figure 8

Absorption spectra of derivatised gold sols reacted with different fusion proteins and constructs. Blue solid and dashed lines show absorption spectra of GNP-GST-NL derivatised gold sol reacted with GST-SNAP25 and SNAP25 respectively. Red solid and dashed lines show absorption spectra of GNP-GST-SNAP25 derivatised gold sol reacted with GST-NL and NL respectively. Turquoise solid and dashed lines show absorption spectra of GNP-GST derivatised gold sol reacted with GST-SNAP25 and GST-NL respectively. Dark yellow solid and dashed lines show absorption spectra of GNP-GST derivatised gold sol reacted with SNAP25 and NL peptides respectively. Dotted red line show absorption spectrum of the GNP-GST-SNAP25 derivatized gold sol incubated with GST protein alone. Dotted blue line show absorption spectrum of the GNP-GST-NL derivatised gold sol incubated with GST protein alone. Dotted black line show absorption spectrum of the GNP-GST derivatised gold sol incubated with GST protein alone. Schematic images of the derivatised GNPs and the colour coding are the same as in Fig. 7. The insert (top right corner) shows blown up section of the absorption spectra to illustrate the two highly similar groups of GNPs identified.