Morphological and structural changes induced by rhodium (II) citrate (Rh
) in 4T1 breast carcinoma cell line after 48 hours of treatment. Cells were incubated with 500 μM Rh2(H2cit)4 for 48 hours and examined by phase contrast microscopy (A, B) and transmission electron microscopy (C-F). (A, C and E) control (cells without treatment); (B, D and F) cells treated with 500 μM of Rh2(H2cit)4. Differences were observed in cell morphology, vacuole amount and mitochondrial condensation between untreated cells (A, C and E) and Rh2(H2cit)4 treated cells (B, D and F). Legends: blebbing (arrows), vacuoles (arrow heads), nucleus (n), mitochondria (m), condensed chromatin (*).