Figure 9
Cytotoxic effect of maghemite nanoparticles associated with rhodium (II) citrate (Magh-Rh 2 (H 2 cit) 4 ) and magnetoliposomes (Lip-Magh-Rh 2 (H 2 cit) 4 ) in breast carcinoma cell lines (MCF-7 and 4T1) and breast normal cell line (MCF-10A). Cells were incubated with free rhodium (II) citrate (Rh2(H2cit)4), Magh-Rh2(H2cit)4 (final concentration: 3 × 1015 iron particles/mL and 23 mM of iron) or Lip-Magh-Rh2(H2cit)4 (final concentration: 12.5 × 1015 iron particles/mL and 94.5 mM of iron) for 24, 48 and 72 h. In all treatments the concentration of Rh2(H2cit)4 used was 50 μM. Data were normalized with the control (cells without treatment) and expressed as mean ± standard error of two independent experiments performed in triplicates. Different letters indicate statistical difference within each treatment (p < 0.05).