Figure 3

Cytotoxicity assays of individual nanocomplex components. A, Cultured Karpas 299 cells were treated for 48 hours with the individual components of the nanocomplex at their maximal concentrations: 100 nM CD30 aptamer, 100 nM ALK siRNA, and 4.2 μM sodium citrate, or vehicle only for the control group. Cell viability (%) was evaluated by flow cytometry using forward scatter (FSC) and side scatter (SSC) parameters as indicated. B, Karpas 299 cells were treated with PEI at concentrations of 5.48 and 1.10 μg/ml for 48 hours, and viable cells were quantified by flow cytometry, as above. C, Cell viability studies using serially diluted PEI ranging from 0.027 to 5.48 μg/ml.