Figure 6

In vivo fluorescence images of tumor accumulation and tissue distribution for FMNPs-BRCAA1 nanoparticles in MGC803 human gastric tumor-bearing athymic nude mice. A, In vivo fluorescence images of athymic nude mice bearing. MGC803 human gastric tumor was obtained after injection of FMNPs-BRCAA1 nanoparticles at different time point. The tumor location is specified with an arrow. A-1: 0 h, A-2:0.5 h, A-3:1 h, A-4:3 h, A-5:6 h, A-6:12 h. B, TBR [Tissue to background (muscle) ratio] value. The TBR value was determined as follows: TBR = (Tumor signal-background signal)/(background signal). C, Ex vivo fluorescence images of dissected organs and tumor of mice bearing MGC803 human gastric tumor sacrificed at 12 h after injection of FMNPs-BRCAA1 nanoparticles. The fluorescence images of dissected organs and tumor were obtained using a fluorescence imaging technique with a 630 nm emission filter. D, Biodistribution of anti- BRCAA1-FMNPs in mice after intravenous injection. Several time points after injection, iron amounts in tissue samples were evaluated by ICP mass spectrometry (n = 3).