Figure 3
Paxillin-QD conjugates associate with newly formed focal adhesion complexes and are released once the complexes are disassembled. Xenopus embryos were injected at the 2-cell stage with the probe (DnaE IC-QDot525 or 585) and RNA encoding Paxillin-EGFP-DnaE IN. Animal cap cells were dissociated from stage 8 Xenopus embryos, induced with activin, and plated onto fibronectin coated slides. (A) Time lapse images (time-interval: 30 sec) show paxillin-QD conjugates associating with newly formed focal adhesion complexes at the filopodia and lamellipodia of mesodermal cells during their migration on fibronectin substrates (see arrows). (B) Time lapse images (time-interval: 10 sec) show paxillin-QD conjugates being released from focal adhesion complexes as they disassemble during migration of mesodermal cells on fibronectin substrates (see arrows).