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Table 3 Fluorescence lifetime imaging microscopy (FLIM) values obtained from cells transfected with GFP-endo and GFP and exposed to SWCNTs.

From: Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration

GFP-endo (+) "Short" (145 ± 17 nm) SWCNTs
  τ1 A1 τ2 A2 τm χ2
Control 1732 ±
65
0.574 ± 0.081 2784 ±
76
0.426 ± 0.074 2128 ±
37
1.200 ± 0.037
5 min. 1588 ± 117 0.601 ± 0.105 2757 ± 116 0.399 ± 0.068 1982 ±
56
1.291 ± 0.088
25 min. 1918 ± 124 0.553 ± 0.109 2560 ± 101 0.447 ± 0.104 2176 ±
58
1.346 ± 0.072
GFP (+) "Short" (145 ± 17 nm) SWCNTs
  τ 1 A 1 τ 2 A 2 τ m χ 2
Control 1638 ± 83 0.558 ± 0.133 2803 ± 107 0.442 ± 0.125 2098 ±
75
1.261 ± 0.082
5 min. 1306 ± 146 0.624 ± 0.173 3054 ± 136 0.376 ± 0.132 1959 ±
98
1.364 ± 0.206
25 min. 1574 ± 186 0.568 ± 0.163 2527 ± 118 0.432 ± 0.138 1947 ±
86
1.342 ± 0.099
GFP-endo (+) "Long" (1.25 ± 0.75 μm) SWCNTs
  τ 1 A 1 τ 2 A 2 τ m χ 2
Control 1945 ± 131 0.550 ± 0.111 2606 ± 107 0.450 ± 0.109 2230 ±
60
1.231 ± 0.063
5 min. 2026 ± 167 0.534 ± 0.110 2515 ± 121 0.466 ± 0.101 2227 ±
70
1.456 ± 0.101
25 min. 1827 ± 151 0.583 ± 0.097 2639 ± 111 0.417 ± 0.088 2137 ±
59
1.337 ± 0.064