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Table 3 Fluorescence lifetime imaging microscopy (FLIM) values obtained from cells transfected with GFP-endo and GFP and exposed to SWCNTs.

From: Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration

GFP-endo (+) "Short" (145 ± 17 nm) SWCNTs

 

τ1

A1

τ2

A2

τm

χ2

Control

1732 ±

65

0.574 ± 0.081

2784 ±

76

0.426 ± 0.074

2128 ±

37

1.200 ± 0.037

5 min.

1588 ± 117

0.601 ± 0.105

2757 ± 116

0.399 ± 0.068

1982 ±

56

1.291 ± 0.088

25 min.

1918 ± 124

0.553 ± 0.109

2560 ± 101

0.447 ± 0.104

2176 ±

58

1.346 ± 0.072

GFP (+) "Short" (145 ± 17 nm) SWCNTs

 

τ 1

A 1

τ 2

A 2

τ m

χ 2

Control

1638 ± 83

0.558 ± 0.133

2803 ± 107

0.442 ± 0.125

2098 ±

75

1.261 ± 0.082

5 min.

1306 ± 146

0.624 ± 0.173

3054 ± 136

0.376 ± 0.132

1959 ±

98

1.364 ± 0.206

25 min.

1574 ± 186

0.568 ± 0.163

2527 ± 118

0.432 ± 0.138

1947 ±

86

1.342 ± 0.099

GFP-endo (+) "Long" (1.25 ± 0.75 μm) SWCNTs

 

τ 1

A 1

τ 2

A 2

τ m

χ 2

Control

1945 ± 131

0.550 ± 0.111

2606 ± 107

0.450 ± 0.109

2230 ±

60

1.231 ± 0.063

5 min.

2026 ± 167

0.534 ± 0.110

2515 ± 121

0.466 ± 0.101

2227 ±

70

1.456 ± 0.101

25 min.

1827 ± 151

0.583 ± 0.097

2639 ± 111

0.417 ± 0.088

2137 ±

59

1.337 ± 0.064