Table 3 Fluorescence lifetime imaging microscopy (FLIM) values obtained from cells transfected with GFP-endo and GFP and exposed to SWCNTs.
From: Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration
GFP-endo (+) "Short" (145 ± 17 nm) SWCNTs | ||||||
|---|---|---|---|---|---|---|
|  | τ1 | A1 | τ2 | A2 | τm | χ2 |
Control | 1732 ± 65 | 0.574 ± 0.081 | 2784 ± 76 | 0.426 ± 0.074 | 2128 ± 37 | 1.200 ± 0.037 |
5 min. | 1588 ± 117 | 0.601 ± 0.105 | 2757 ± 116 | 0.399 ± 0.068 | 1982 ± 56 | 1.291 ± 0.088 |
25 min. | 1918 ± 124 | 0.553 ± 0.109 | 2560 ± 101 | 0.447 ± 0.104 | 2176 ± 58 | 1.346 ± 0.072 |
GFP (+) "Short" (145 ± 17 nm) SWCNTs | ||||||
|  | τ 1 | A 1 | τ 2 | A 2 | τ m | χ 2 |
Control | 1638 ± 83 | 0.558 ± 0.133 | 2803 ± 107 | 0.442 ± 0.125 | 2098 ± 75 | 1.261 ± 0.082 |
5 min. | 1306 ± 146 | 0.624 ± 0.173 | 3054 ± 136 | 0.376 ± 0.132 | 1959 ± 98 | 1.364 ± 0.206 |
25 min. | 1574 ± 186 | 0.568 ± 0.163 | 2527 ± 118 | 0.432 ± 0.138 | 1947 ± 86 | 1.342 ± 0.099 |
GFP-endo (+) "Long" (1.25 ± 0.75 μm) SWCNTs | ||||||
|  | τ 1 | A 1 | τ 2 | A 2 | τ m | χ 2 |
Control | 1945 ± 131 | 0.550 ± 0.111 | 2606 ± 107 | 0.450 ± 0.109 | 2230 ± 60 | 1.231 ± 0.063 |
5 min. | 2026 ± 167 | 0.534 ± 0.110 | 2515 ± 121 | 0.466 ± 0.101 | 2227 ± 70 | 1.456 ± 0.101 |
25 min. | 1827 ± 151 | 0.583 ± 0.097 | 2639 ± 111 | 0.417 ± 0.088 | 2137 ± 59 | 1.337 ± 0.064 |