Visualized PNA on DNA strands. a) The fluorescently labelled PNA can be seen as green dots. Their mutual distance on the DNA-construct is 2.45 μm and they are located on the predicted positions on the DNA-strands. Red circles are the spots, serving as DNA-anchors. b) Four of the relative distances of the detected PNA-lables are highlighted. Their relative positions fit quite well to the theoretical predictions derived from the DNA-construct.