Figure 9

Test for construct orientation. a) L represents the successful binding situation shown in figure 8 - Cy5-cF6 was prehybridized whereas LcF5-Btn is located in the corresponding spot whose signal is plotted here - R: catcher oligonucleotides for the right side, which cannot bind to the DNA strand because of the prehybridization with Cy5-cF6 - b) the opposite end was prehybridized with Cy3-cF5 so that the whole ensemble should only bind in the opposite direction.