Figure 4
From: Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays
Single sequence exposure gradients with different oxidation protocols. Signal intensity is from a hybridized, Cy3-labeled complementary sequence. Black circles: single oxidation after last coupling. Red squares: an oxidation at the middle and one at the end. Green triangles: oxidation after every fourth coupling and after the final coupling. Blue downwards triangles: Oxidation after every base. Insert graph: no oxidation steps. Inset images: one of four replicate gradients on the array with a middle and terminal oxidation; exposure increases bottom to top and right to left.