Table 1 Synthesis protocols
From: Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays
Cycle T | Cycle M (Cy3) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Function | Mode | pulse | sec | Description | Function | Mode | pulses | sec | Description |
$Coupling | Â | Â | Â | Â | $Coupling | Â | Â | Â | Â |
1/*Wsh | */PULSE | 20 | 0 | "Flush system with Wsh" | 1/*Wsh | */PULSE | 20 | 0 | "Flush system with Wsh" |
2/*Act | */PULSE | 6 | 0 | "Act" | 2/*Act | */PULSE | 6 | 0 | "Act" |
21/*T + Act | */PULSE | 5 | 0 | "T + Act" | 25/*8 + Act | */PULSE | 5 | 0 | "8 + Act" |
2/*Act | */PULSE | 8 | 0 | "Chase with Act" | 2/*Act | */PULSE | 8 | 0 | "Chase with Act" |
1/*Wsh | */PULSE | 3 | 60 | "Couple monomer" | 1/*Wsh | */PULSE | 3 | 300 | "Couple monomer" |
1/*Wsh | */PULSE | 10 | 0 | "Flush system with Wsh" | 1/*Wsh | */PULSE | 20 | 0 | "Flush system with Wsh" |
$Capping | Â | Â | Â | Â | 1/*Wsh | */PULSE | 10 | 0 | "Flush system with Wsh" |
13/*Caps | */PULSE | 10 | 0 | "Caps" | 2/*Act | */PULSE | 6 | 0 | "Act" |
13/*Caps | */PULSE | 10 | 120 | "Caps" | 25/*8 + Act | */PULSE | 5 | 0 | "8 + Act" |
12/*Wsh A | */PULSE | 25 | 60 | "Wsh A" | 2/*Act | */PULSE | 8 | 0 | "Chase with Act" |
12/*Wsh A | */PULSE | 30 | 0 | "Wsh A" | 1/*Wsh | */PULSE | 3 | 300 | "Couple monomer" |
40/*Gas A | */PULSE | 1 | 30 | "Dry column" | 1/*Wsh | */PULSE | 10 | 0 | "Flush system with Wsh" |
$Oxidizing | Â | Â | Â | Â | $Oxidizing | Â | Â | Â | Â |
15/*Ox | */PULSE | 30 | 0 | "Oxidize" | 15/*Ox | */PULSE | 30 | 0 | "Oxidize" |
12/*Wsh A | */PULSE | 45 | 0 | "Flush system with Wsh A" | 12/*Wsh A | */PULSE | 300 | 0 | "Wsh A" |
17/*Aux | */PULSE | 45 | 0 | "Aux" | 12/*Wsh A | */PULSE | 300 | 300 | "Wsh A" |
130/*Event 2 Out | */NA | 4 | 3 | "Event 2 Out" | Â | Â | Â | Â | Â |
17/*Aux | */PULSE | 16 | 60 | "Aux" | Â | Â | Â | Â | Â |
12/*Wsh A | */PULSE | 25 | 0 | "Flush system with Wsh A" | Â | Â | Â | Â | Â |