Cyclic voltammetric integration of different multilayer assemblies. 7a) Cyclic voltammetry of 1, 2, 3, 4-bilayer assemblies of cyt c/SiNPs (Ø = 5 nm) and a cyt c monolayer (M) Au-MU/MUA-cyt c for comparison. Inset: 7b) Electrochemical determined concentration of cytochrome c in the multilayer assemblies [1, 2, 3, 4-bilayers SiNPs (Ø = 5 nm)/cyt c], compared to a monolayer (M). Inset: 7c) Peak separation of cyt c multilayer assemblies determined by cyclic voltammetry for 1, 2, 3, 4-bilayers of SiNPs/cyt c assembled on a monolayer (M). For the data represent in this figure a scan rate of 100 mV/s, KPP pH 7 was used.