Figure 5
Cytotoxic effect of the NIR fluorescent P(EF-PLLA) nanoparticles on human colorectal adenocarcinoma LS174t, SW480 and HT29 cell lines measured by the LDH assay. Cells (3 × 105) were incubated for 24 and 48 h with the P(EF-PLLA) nanoparticles (1.25 and 2.5 mg/mL in PBS). Cells were incubated with 1% Triton-x-100 as positive control (100% toxicity). In addition, cells were incubated with Triton-x-100 1% and the P(EF-PLLA) nanoparticles (2.5 mg/mL) to revoke any interaction of the nanoparticles with the LDH kit components. Untreated cells (negative control) were similarly incubated. Each bar represents mean ± standard deviations of 4 separate samples.