Figure 3

Evaluation of efficient and gentle cell manipulation parameters. Normalized fluorescence levels of delivered 10 kDa FITC dextran into ZMTH3 cells (left), the cell viability (middle) and the efficiency ratio (normalized ratio of FITC fluorescence level and viability) for different manipulation parameters in dependence of radiant exposure (20 mJ/cm² to 140 mJ/cm²). A) Different scanning velocities at a defined AuNP concentration of 11 μg/ml and particle size of 250 nm, B) particle concentrations at a defined scanning velocity of 50 mm/s and particle size of 250 nm and C) different particle sizes (80 nm, 150 nm, 200 nm, 250 nm) at a defined AuNP concentration of 11 μg/ml and scanning velocity of 50 mm/s. D) Viability of cells incubated with AuNP for a time span up to 72 h after treatment with different radiant exposures (left) and different AuNP concentrations without laser exposure (right). The respective data points represents the mean value ± standard error of n = 4 independent experiments in triplicates on different days.