Figure 5

Ishikawa cells grown on bioimprinted substrates for 48 hours, cells are stained with Commassie blue. Left image, unannotated. Right image, the bioimprint features are outlined in grey, the secondary cell culture (dark blue) is outlined in yellow. Arrows (red) note regions of the cell monolayer that peeled off and folded back. This figure illustrates that the growth of the secondary cell culture has been guided by the footprint of the replicated cell patterns.