Table 1 Phantom and animal tests in this study
| Test type | Test context |
|---|---|
| Phantom test |
• Anti-AFP reagent:
0.25 g in volume and 0.3, 0.2, 0.1, 0.01, 0.005 emu/g in concentration. |
|
• Phantoms:
Micro test tubes were filled with anti-AFP reagent. | |
|
• Imaging:
The fused images composed of the magnetic image for the spatial distribution of anti-AFP MNPs and the optic image of micro test tubes. | |
| Animal tests |
• Dose of Anti-AFP reagent:
0.9 g in volume and 0.3 emu/g in concentration. |
|
• Tumor rats:
Liver tumors were implanted on backs for 2 rats and in livers for 3 rats, separately. | |
| Anti-AFP reagents were intravenously injected through tail veins. | |
|
• Imaging:
The 2 tumor rats with back tumors were imaged in a prone position by using the dual-model SSB at 0 h. | |
| The 3 tumor rats implanted with liver tumors were imaged in a supine position at 0 h, 24 h, and with their bellies opened at 24.5 h. | |
| Both the stacked block and separated lobe livers of the 3 tumor rats implanted with liver tumors were magnetically examined ex vivo. | |
|
• Tissue stain:
After identifying the red-spot and no-spot regions in separated lobe livers by this dual-model SSB, small pieces of liver tissue in both regions for HE stain and AFP stain were performed. |