Figure 6

Analysis of cytoskeleton. (A) Representative images of cells immunostained for α-tubulin (green) and DNA counterstained with Hoechst-33258 (blue). (a) Interphase control cells. (a’) Metaphase control cell. (b-b’) Interphase cells incubated for 24 h with DMSA-SPION and observed by fluorescence and bright-field microscopy, respectively; (c-c’) cells incubated for 48 h; (d-d’) cells incubated for 72 h. (e-e’) Mitotic spindle of cells incubated for 24 h, (f-f’) 48 h and (g-g’) 72 h. (B) Merged images of F-actin labeled with rhodamine-phalloidin (red), vinculin immunostaining (green) and DNA counterstained with Hoechst-33258 (blue). (a) Control untreated cells. (b-b’) Cells treated with DMSA-SPION for 24 h and observed by fluorescence and bright-field microscopy, respectively. (c-c’) Cells incubated for 48 h. (d-d’) Cells incubated for 72 h. Scale bar 10 μm.