Figure 5

Intracellular location of MNPs on hVM cells. A-B. hVM cells were incubated with 50 nm (A) and 100 nm MNPs-Cy3 (B) at 50 μg/ml for 72 h. After fixation, cells were stained with Phalloidin A488 (green). MNPs in A were detected by staining with an anti-dextran antibody (red). Orthogonal projections are shown in A and B, confirming the colocalization of MNPs and phalloidin. C-E. To determine the final intracellular localization of MNPs-Cy3, hVM cells were incubated with 100 nm MNPs-Cy3 at 50 μg/ml for 72 h, antibodies anti-EEA1 specific for early endosomes (C), anti-mannosidase II specific for Golgi apparatus (D) and anti-CD63 specific for lysosomes (E), were used. Nuclei were counterstained with ToPro3. Scale bars: 10 μm in A-B and 15 μm in C-E.