Figure 1

UV-Visible absorption spectra of invertase-onion membranes. (a) UV-Visible absorption spectra of invertase, blank onion membrane, AuCl₄ solution and onion membrane with INV and AuCl₄ incubated for 96 h in 20 mM acetate buffer solution (pH 5.0). (b) Periodical UV-visible absorption spectra of invertase-onion membrane with dispersed nanogold in 20 mM acetate buffer solution (pH 5.0). Inset I: Absorption peak at 301 nm; Inset II: Absorption peak at 540 nm. (c) Plot of time versus absorption of nanogold clusters synthesized on an invertase-onion membrane at 301 nm (a) and 540 nm (b), respectively.