Figure 11

Effect of free and conjugated FGF2 on osteogenic differentiation of hBM-MSCs. Cells were grown in osteogenic differentiation media (A, control) supplemented with 10 ng/ml free FGF2 (C) or conjugated FGF2 (D) or free IO/HSA NPs (B), for 14 days. Cells were stained with Alizarin-red. Bar-200μm. (E) To evaluate the osteogenic differentiation capacity of each supplement, the Alizarin-red O positive area/total area x100 was calculated in 3 areas from triplicate samples for control (c) or each supplement at 3 or 10 ng/ml as indicated in the graph.