Figure 7

Determination of uptake of Cy7-IO/HSA NPs by hBM-MSCs using Prussian Blue iron staining. Cells were grown in the absence (control, J) or presence of 10 ng/ml (A,C), 50 ng/ml (D,F)or 100 ng/ml (G,I) free FGF2 (A, D, G) or conjugated-FGF2 NPs (C, F, I) , or non-conjugated IO/HSA NPs at concentration of 9 μg/ml (B), 45 μg/ml (E) or 90 μg/ml (H). Following fixation in 4% PFA, cells were stained with Prussian Blue iron stain (blue) and counter stained with Nuclear Fast Red (red). The percentage of Prussian Blue positive cells was calculated from 3 microscopic fields and is indicated at the bottom of each picture. Magnification (×200).