Table 1 Properties of PCT Nbs from the naïve library and the immune library [23]
VHH | M W a (kDa) | pI b | ε c (g/L) | Yield (mg/L) | K on (M -1 s -1 ) | K Off (s -1 ) | K D (M) |
|---|---|---|---|---|---|---|---|
PCT Nb1 | 15.47 | 6.03 | 1.964 | 7.5 | 4.16 × 104 | 5.10 × 10-4 | 1.23 × 10-8 |
PCT Nb2 | 15.18 | 8.73 | 1.833 | 4.5 | 1.47 × 105 | 1.97 × 10-4 | 1.34 × 10-9 |
PCT Nb3 | 16.08 | 7.19 | 1.954 | 6.0 | 4.31 × 104 | 3.83 × 10-4 | 8.89 × 10-9 |
PCT Nb20 | 16.05 | 6.13 | 2.498 | 7.1 | 2.85 × 104 | 9.52 × 10-4 | 3.34 × 10-8 |
PCT Nb45 | 15.71 | 7.95 | 2.027 | 4.9 | 1.64 × 103 | 4.02 × 10-5 | 2.45 × 10-8 |
PCT Nb50 | 16.12 | 7.17 | 2.577 | 8.7 | 2.39 × 104 | 6.34 × 10-4 | 2.65 × 10-9 |
PCT Nb52 | 15.59 | 6.70 | 2.367 | 9.3 | 2.32 × 104 | 6.10 × 10-4 | 2.63 × 10-8 |
PCT Nb85 | 16.07 | 7.17 | 2.578 | 6.4 | 2.11 × 104 | 8.21 × 10-4 | 3.89 × 10-9 |
PCT Nb86 | 16.18 | 8.36 | 2.317 | 5.5 | 2.52 × 104 | 8.65 × 10-4 | 3.43 × 10-9 |
PCT Nb87 | 16.64 | 6.38 | 1.825 | 10.3 | 4.18 × 105 | 2.58 × 10-4 | 6.17 × 10-9 |
PCT Nb88 | 15.63 | 8.80 | 1.925 | 7.6 | 1.99 × 104 | 8.05 × 10-4 | 4.04 × 10-9 |