Figure 5

Bactericidal activity of MNP@CSA-13 against P. aeruginosa strains. Bactericidal activity of CSA-13 (0.7 μg/ml), MNPs (4.3 μg/ml) and MNP-CSA-13 (5 μg/ml) against P. aeruginosa PAO14 (CT - control) (panel A). To compare antibacterial potential of MNP, CSA-13 and MNP-CSA-13 against P. aeruginosa, CSA-13 concentration was estimated based on physicochemical data and yield of reaction. Panels B and C show survival of PAO1 and a clinical strain of P aeruginosa isolated from cystic fibrosis sputum in the presence of MNPs (black circles) and MNP-CSA-13 (white circles) respectively. Reduction of P. aeruginosa Xen 5 (white triangels) chemiluminescence signal (~10⁸ CFU/ml) after colistin – COL (100 μg/ml; white square), MNP (100 μg/ml; black square), CSA-13 (100 μg/ml; black circles) and MNP-CSA-13 (100 μg/ml; white circles) addition – panel D. Data represent mean ± SD, n = 3 with * statistical significance compared to untreated bacteria cells (p ≤ 0.05); ^ statistical significance compared to bacteria treated with free CSA-13 (p ≤ 0.2) (panel A).