Figure 11

Hyperspectral fluorescence imaging of QD-BRET (QD) labeled spermatozoa. Negative control spermatozoa (a) were used to remove false positive signals and validation of QD-BRET fluorescence spectral libraries that were captured around or near 655 nm emission wavelength, as indicated by the arrow (b). Hyperspectral images of spermatozoa were taken (a, c, e) followed by the comparison of all pixels with the QD1− and QD1+ spectral libraries (Not shown here). Pixels that matched the spectral are mapped with a pseudo-red color (arrows) illustrating the presence and location of QD1− (d) or QD1+ (f).