Figure 4

In vitro cytotoxicity analysis of Zn-CS/nHAp/β-GP hydrogel. a MTT assay of C3H10T1/2 cells grown for 24 h in medium conditioned with Zn-CS/nHAp/β-GP hydrogel. Wells treated with 0.1% Triton X-100 served as positive controls. Asterisk indicates significant decrease compared with control (p < 0.05) (n = 5). b FDA staining of C3H10T1/2 cells grown in Zn-CS/nHAp/β-GP hydrogel for 5 days and assessed with ×4 and ×10 objectives for cellular morphology. Control cells (I) and cells grown in hydrogel (III) showed highly spread cellular extensions. Magnified images of control and hydrogel-treated cells are depicted in (II) and (IV), respectively.