Multiplexing Au-nanoprobe based detection. DNA extracted from several biological fluids is amplified (e.g. PCR) and then a single Au-nanoprobe is used to detect presence of pathogens. Depicted is the detection of M. tuberculosis and P. falciparum. Map shows the geografical differences of Malaria and TB incidence where the highest rates are found predominantly in low-income countries in Africa and South America. This approach can be transposed into a simple and portable molecular diagnostic platform to be used at peripheral laboratories and/or point-of-need, coupled to a “smartphone” for data analysis and location metadata for real-time epidemiologic data .