Fig. 4
Confocal images of HeLa cells which internalized differently shaped particles (red fluorescence) after incubation for 24 h. a “Small” spherical CaCO3 particles, b “small” ellipsoidal CaCO3 particles, c cuboidal CaCO3 particles, d “big” ellipsoidal CaCO3 particles, and e “big” spherical SiO2 particles (used as control). Particles are labeled with TRITC (red). Nuclei, lysosomes, and cytoskeletons are fluorescence labeled with DAPI (blue), Anti-LAMP1 labeled with DyLight 649 (artificially colored in yellow), and Oregon Green® 488 phalloidin (green), respectively. 1, 2, 3, 4 and 5 mean the red, green, blue and yellow channels and the merged image, respectively. The scale bars in all images correspond to 20 μm.